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cd47 detection  (Miltenyi Biotec)


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    Miltenyi Biotec cd47 detection
    Schematic presentation of different parallel or anti-parallel coiled coil pairing. N5N6 (strong parallel CC pair), P3AP4 (anti-parallel CC pair) and P3SP4S (weak parallel CC pair) ( a ). Diagram showing gRNA positions within exon 2 of the human <t>CD47</t> gene ( b ). Indel mutation quantification by the T7E1 assay, determined 48 h post transfection (HEK293 cells (2*10 5 cells/ml) were transfected with plasmids encoding gRNA and CCExo) for several tested gRNAs ( c – h ), a combination of all gRNAs ( i ), and gRNA1 in combination with CCExo containing coiled-coils with increasing affinity (P3P4 < N5N6) ( j ). Data present three individual separate experiments ( n = 3). * P < 0.05, **< 0.01, ***< 0.001 and **** P < 0.0001. All P values are from ordinary one-way ANOVA followed by Tukey’s multiple comparisons test compared to Cas9 values or if stated otherwise by brackets. Data are presented as mean values ± SEM as appropriate ( c – j ). Expression of Cas9 or its variants in HEK293 cells 48 h post transfection determined by western blot and immunodetection using specific anti-Cas9 antibodies. α-tubulin was used as a loading control. A representative blot from two individual separate experiment is shown ( k ).
    Cd47 Detection, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd47+detection/pmc09226073-361-1-9?v=Miltenyi+Biotec
    Average 93 stars, based on 8 article reviews
    cd47 detection - by Bioz Stars, 2026-08
    93/100 stars

    Images

    1) Product Images from "Coiled-coil heterodimer-based recruitment of an exonuclease to CRISPR/Cas for enhanced gene editing"

    Article Title: Coiled-coil heterodimer-based recruitment of an exonuclease to CRISPR/Cas for enhanced gene editing

    Journal: Nature Communications

    doi: 10.1038/s41467-022-31386-1

    Schematic presentation of different parallel or anti-parallel coiled coil pairing. N5N6 (strong parallel CC pair), P3AP4 (anti-parallel CC pair) and P3SP4S (weak parallel CC pair) ( a ). Diagram showing gRNA positions within exon 2 of the human CD47 gene ( b ). Indel mutation quantification by the T7E1 assay, determined 48 h post transfection (HEK293 cells (2*10 5 cells/ml) were transfected with plasmids encoding gRNA and CCExo) for several tested gRNAs ( c – h ), a combination of all gRNAs ( i ), and gRNA1 in combination with CCExo containing coiled-coils with increasing affinity (P3P4 < N5N6) ( j ). Data present three individual separate experiments ( n = 3). * P < 0.05, **< 0.01, ***< 0.001 and **** P < 0.0001. All P values are from ordinary one-way ANOVA followed by Tukey’s multiple comparisons test compared to Cas9 values or if stated otherwise by brackets. Data are presented as mean values ± SEM as appropriate ( c – j ). Expression of Cas9 or its variants in HEK293 cells 48 h post transfection determined by western blot and immunodetection using specific anti-Cas9 antibodies. α-tubulin was used as a loading control. A representative blot from two individual separate experiment is shown ( k ).
    Figure Legend Snippet: Schematic presentation of different parallel or anti-parallel coiled coil pairing. N5N6 (strong parallel CC pair), P3AP4 (anti-parallel CC pair) and P3SP4S (weak parallel CC pair) ( a ). Diagram showing gRNA positions within exon 2 of the human CD47 gene ( b ). Indel mutation quantification by the T7E1 assay, determined 48 h post transfection (HEK293 cells (2*10 5 cells/ml) were transfected with plasmids encoding gRNA and CCExo) for several tested gRNAs ( c – h ), a combination of all gRNAs ( i ), and gRNA1 in combination with CCExo containing coiled-coils with increasing affinity (P3P4 < N5N6) ( j ). Data present three individual separate experiments ( n = 3). * P < 0.05, **< 0.01, ***< 0.001 and **** P < 0.0001. All P values are from ordinary one-way ANOVA followed by Tukey’s multiple comparisons test compared to Cas9 values or if stated otherwise by brackets. Data are presented as mean values ± SEM as appropriate ( c – j ). Expression of Cas9 or its variants in HEK293 cells 48 h post transfection determined by western blot and immunodetection using specific anti-Cas9 antibodies. α-tubulin was used as a loading control. A representative blot from two individual separate experiment is shown ( k ).

    Techniques Used: Mutagenesis, Transfection, Expressing, Western Blot, Immunodetection

    HEK293 cells (2*10 5 cells/ml) were transfected with plasmids encoding target gRNA and CCExo. Genomic DNA was isolated 48 h post transfection, the targeted region PCR amplified and subjected to NGS analysis. NGS analysis was performed for 200 bp amplicons for gRNA targeting MYD88 gene ( a ) or 400 bp amplicons for gRNA targeting CD47 gene ( b ), VEGFα gene ( c ) or EMX1 gene ( d ). Diagrams are showing percentage (calculated based on number of total mutated reads) of deletions (Δ) of various lengths. Genome wide determinations of potential off-target activities of CCExo for VEGFα sites in HEK293 cells and for TRBC1 site in human K562 cells, predicted by CIRCLE-seq ( e ).
    Figure Legend Snippet: HEK293 cells (2*10 5 cells/ml) were transfected with plasmids encoding target gRNA and CCExo. Genomic DNA was isolated 48 h post transfection, the targeted region PCR amplified and subjected to NGS analysis. NGS analysis was performed for 200 bp amplicons for gRNA targeting MYD88 gene ( a ) or 400 bp amplicons for gRNA targeting CD47 gene ( b ), VEGFα gene ( c ) or EMX1 gene ( d ). Diagrams are showing percentage (calculated based on number of total mutated reads) of deletions (Δ) of various lengths. Genome wide determinations of potential off-target activities of CCExo for VEGFα sites in HEK293 cells and for TRBC1 site in human K562 cells, predicted by CIRCLE-seq ( e ).

    Techniques Used: Transfection, Isolation, Amplification, Genome Wide



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    Schematic presentation of different parallel or anti-parallel coiled coil pairing. N5N6 (strong parallel CC pair), P3AP4 (anti-parallel CC pair) and P3SP4S (weak parallel CC pair) ( a ). Diagram showing gRNA positions within exon 2 of the human <t>CD47</t> gene ( b ). Indel mutation quantification by the T7E1 assay, determined 48 h post transfection (HEK293 cells (2*10 5 cells/ml) were transfected with plasmids encoding gRNA and CCExo) for several tested gRNAs ( c – h ), a combination of all gRNAs ( i ), and gRNA1 in combination with CCExo containing coiled-coils with increasing affinity (P3P4 < N5N6) ( j ). Data present three individual separate experiments ( n = 3). * P < 0.05, **< 0.01, ***< 0.001 and **** P < 0.0001. All P values are from ordinary one-way ANOVA followed by Tukey’s multiple comparisons test compared to Cas9 values or if stated otherwise by brackets. Data are presented as mean values ± SEM as appropriate ( c – j ). Expression of Cas9 or its variants in HEK293 cells 48 h post transfection determined by western blot and immunodetection using specific anti-Cas9 antibodies. α-tubulin was used as a loading control. A representative blot from two individual separate experiment is shown ( k ).
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    Schematic presentation of different parallel or anti-parallel coiled coil pairing. N5N6 (strong parallel CC pair), P3AP4 (anti-parallel CC pair) and P3SP4S (weak parallel CC pair) ( a ). Diagram showing gRNA positions within exon 2 of the human <t>CD47</t> gene ( b ). Indel mutation quantification by the T7E1 assay, determined 48 h post transfection (HEK293 cells (2*10 5 cells/ml) were transfected with plasmids encoding gRNA and CCExo) for several tested gRNAs ( c – h ), a combination of all gRNAs ( i ), and gRNA1 in combination with CCExo containing coiled-coils with increasing affinity (P3P4 < N5N6) ( j ). Data present three individual separate experiments ( n = 3). * P < 0.05, **< 0.01, ***< 0.001 and **** P < 0.0001. All P values are from ordinary one-way ANOVA followed by Tukey’s multiple comparisons test compared to Cas9 values or if stated otherwise by brackets. Data are presented as mean values ± SEM as appropriate ( c – j ). Expression of Cas9 or its variants in HEK293 cells 48 h post transfection determined by western blot and immunodetection using specific anti-Cas9 antibodies. α-tubulin was used as a loading control. A representative blot from two individual separate experiment is shown ( k ).
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    Image Search Results


    Schematic presentation of different parallel or anti-parallel coiled coil pairing. N5N6 (strong parallel CC pair), P3AP4 (anti-parallel CC pair) and P3SP4S (weak parallel CC pair) ( a ). Diagram showing gRNA positions within exon 2 of the human CD47 gene ( b ). Indel mutation quantification by the T7E1 assay, determined 48 h post transfection (HEK293 cells (2*10 5 cells/ml) were transfected with plasmids encoding gRNA and CCExo) for several tested gRNAs ( c – h ), a combination of all gRNAs ( i ), and gRNA1 in combination with CCExo containing coiled-coils with increasing affinity (P3P4 < N5N6) ( j ). Data present three individual separate experiments ( n = 3). * P < 0.05, **< 0.01, ***< 0.001 and **** P < 0.0001. All P values are from ordinary one-way ANOVA followed by Tukey’s multiple comparisons test compared to Cas9 values or if stated otherwise by brackets. Data are presented as mean values ± SEM as appropriate ( c – j ). Expression of Cas9 or its variants in HEK293 cells 48 h post transfection determined by western blot and immunodetection using specific anti-Cas9 antibodies. α-tubulin was used as a loading control. A representative blot from two individual separate experiment is shown ( k ).

    Journal: Nature Communications

    Article Title: Coiled-coil heterodimer-based recruitment of an exonuclease to CRISPR/Cas for enhanced gene editing

    doi: 10.1038/s41467-022-31386-1

    Figure Lengend Snippet: Schematic presentation of different parallel or anti-parallel coiled coil pairing. N5N6 (strong parallel CC pair), P3AP4 (anti-parallel CC pair) and P3SP4S (weak parallel CC pair) ( a ). Diagram showing gRNA positions within exon 2 of the human CD47 gene ( b ). Indel mutation quantification by the T7E1 assay, determined 48 h post transfection (HEK293 cells (2*10 5 cells/ml) were transfected with plasmids encoding gRNA and CCExo) for several tested gRNAs ( c – h ), a combination of all gRNAs ( i ), and gRNA1 in combination with CCExo containing coiled-coils with increasing affinity (P3P4 < N5N6) ( j ). Data present three individual separate experiments ( n = 3). * P < 0.05, **< 0.01, ***< 0.001 and **** P < 0.0001. All P values are from ordinary one-way ANOVA followed by Tukey’s multiple comparisons test compared to Cas9 values or if stated otherwise by brackets. Data are presented as mean values ± SEM as appropriate ( c – j ). Expression of Cas9 or its variants in HEK293 cells 48 h post transfection determined by western blot and immunodetection using specific anti-Cas9 antibodies. α-tubulin was used as a loading control. A representative blot from two individual separate experiment is shown ( k ).

    Article Snippet: For CD47 detection we used human CD47-FITC antibodies from Miltenyi Biotec (130-101-344).

    Techniques: Mutagenesis, Transfection, Expressing, Western Blot, Immunodetection

    HEK293 cells (2*10 5 cells/ml) were transfected with plasmids encoding target gRNA and CCExo. Genomic DNA was isolated 48 h post transfection, the targeted region PCR amplified and subjected to NGS analysis. NGS analysis was performed for 200 bp amplicons for gRNA targeting MYD88 gene ( a ) or 400 bp amplicons for gRNA targeting CD47 gene ( b ), VEGFα gene ( c ) or EMX1 gene ( d ). Diagrams are showing percentage (calculated based on number of total mutated reads) of deletions (Δ) of various lengths. Genome wide determinations of potential off-target activities of CCExo for VEGFα sites in HEK293 cells and for TRBC1 site in human K562 cells, predicted by CIRCLE-seq ( e ).

    Journal: Nature Communications

    Article Title: Coiled-coil heterodimer-based recruitment of an exonuclease to CRISPR/Cas for enhanced gene editing

    doi: 10.1038/s41467-022-31386-1

    Figure Lengend Snippet: HEK293 cells (2*10 5 cells/ml) were transfected with plasmids encoding target gRNA and CCExo. Genomic DNA was isolated 48 h post transfection, the targeted region PCR amplified and subjected to NGS analysis. NGS analysis was performed for 200 bp amplicons for gRNA targeting MYD88 gene ( a ) or 400 bp amplicons for gRNA targeting CD47 gene ( b ), VEGFα gene ( c ) or EMX1 gene ( d ). Diagrams are showing percentage (calculated based on number of total mutated reads) of deletions (Δ) of various lengths. Genome wide determinations of potential off-target activities of CCExo for VEGFα sites in HEK293 cells and for TRBC1 site in human K562 cells, predicted by CIRCLE-seq ( e ).

    Article Snippet: For CD47 detection we used human CD47-FITC antibodies from Miltenyi Biotec (130-101-344).

    Techniques: Transfection, Isolation, Amplification, Genome Wide